<<< Full experiment listing


PXD013891 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMeiosis-associated argonaute (MAGO) proteins are necessary for meiosis in maize
DescriptionPlants do not specify their germline until late in their life cycle. Hence, the plant germline is normally specified from terminally differentiated somatic cells, though the precise mechanism(s) are unknown. We have found that male gametogenesis in maize is associated with the accumulation of distinct 21-nt phased small-interfering RNAs (phasiRNAs) generated by meiosis-associated argonaute (MAGO) proteins. MAGO1 accumulates in the epidermis of pre-meiotic anthers while MAGO2 is found in developing meiocytes. We have found that MAGO proteins are required for meiocyte development as mutants display chromosomal defects and male infertility. Furthermore, we detect the heat stress-induced activation of a distinct class of Long terminal repeat retrotransposons in the male germline of MAGO mutants. Our data suggests that MAGO proteins and the reproductive phasiRNAs play important roles protecting the germline from transposable elements during environmental stress conditions.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrew Bottrill
SpeciesList scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationListPhospho; Oxidation; Acetyl; Carbamidomethyl
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-17 01:36:28ID requested
12022-01-04 02:43:00announced
Publication List
Keyword List
submitter keyword: Argonaute, Meiosis, Maize, Zea mays, Proteomics, Phosphorylation
Contact List
Dr Jose Gutierrez-Marcos
contact affiliationSchool of Life Sciences, University of Warwick, Coventry, United Kingdom
contact emailJ.F.Gutierrez-Marcos@warwick.ac.uk
lab head
Andrew Bottrill
contact affiliationUniversity of Warwick
contact emaila.r.bottrill@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD013891
PRIDE project URI
Repository Record List
[ + ]