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PXD013274 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMarine Proteobacteria metabolise glycolate via the β-hydroxyaspartate cycle
DescriptionOne of the most abundant organic carbon sources in the ocean is glycolate, a compound that is commonly secreted by marine phytoplankton resulting in an estimated annual flux of one petagram of glycolate in marine environments. While it is generally accepted that glycolate is oxidized to glyoxylate by marine bacteria, the further fate of this C2 metabolite is not well understood. Here we show that ubiquitous marine Proteobacteria are able to assimilate glyoxylate via the  hydroxyaspartate cycle (BHAC) that was originally proposed 56 years ago. We unravel the biochemistry of the BHAC and describe the structure of its key enzymes, including a previously unknown primary imine reductase. Overall, the BHAC allows for the direct production of oxaloacetate from glyoxylate through only four enzymatic steps, representing the most efficient glyoxylate assimilation route described to date. Analysis of marine metagenomes shows that the BHAC is globally distributed and on average 20-fold more abundant than the glycerate pathway, the only other known pathway for net glyoxylate assimilation. In a field study on a phytoplankton bloom, we show that glycolate is present in high nanomolar concentrations and taken up by prokaryotes at rates that allow a full turnover of the glycolate pool within one week. During the bloom, the BHAC is present in up to 1.5% of the bacterial community and actively transcribed, supporting its role in glycolate assimilation and suggesting a new trophic interaction between autotrophic phytoplankton and heterotrophic bacterioplankton.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTimo Glatter
SpeciesList scientific name: Paracoccus denitrificans PD1222; NCBI TaxID: 318586;
ModificationListOxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-28 01:10:21ID requested
12019-08-14 04:42:10announced
22019-10-11 05:40:01announced2019-10-11: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Paracoccus denitrificans
Contact List
Tobias Juergen Erb
contact affiliationDepartment of Biochemistry and Synthetic Metabolism, Max Planck Institute for terrestrial Microbiology, Marburg, Germany
contact emailtoerb@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliationMPI Marburg
contact emailglatter@mpi-marburg.mpg.de
dataset submitter
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Dataset FTP location
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