<<< Full experiment listing


PXD013219 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Flemmingsome reveals an ESCRT-to-membrane coupling required for cytokinesis
DescriptionCytokinesis requires the constriction of ESCRT-III filaments on the midbody side, where abscission occurs. After ESCRT recruitment at the midbody, it is not known how the ESCRT-III machinery localizes to the abscission site. We obtained the proteome of intact, post-abscission midbodies (Flemmingsome) and revealed enriched proteins in this organelle. We propose that the ESCRT-III machinery must be physically coupled to a membrane protein at the cytokinetic abscission site for productive scission, revealing novel common requirements in cytokinesis, exosome formation and retroviral budding.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThibaut Douché
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListL-methionine sulfoxide; ubiquitination signature dipeptidyl lysine; phosphorylated residue; N-acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-25 07:18:12ID requested
12020-05-21 23:54:20announced
Publication List
Addi C, Presle A, Fr, é, mont S, Cuvelier F, Rocancourt M, Milin F, Schmutz S, Chamot-Rooke J, Douch, é T, Duchateau M, Giai Gianetto Q, Salles A, M, é, nager H, Matondo M, Zimmermann P, Gupta-Rossi N, Echard A, The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis. Nat Commun, 11(1):1941(2020) [pubmed]
Keyword List
submitter keyword: Human, Cytokinesis, Midbody, ESCRT machinery, QExactive, LC-MS/MS
Contact List
Arnaud Echard
contact affiliationMembrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS, 25–28 rue du Dr Roux, F-75015 Paris, France
contact emailarnaud.echard@pasteur.fr
lab head
Thibaut Douché
contact affiliationInstitut Pasteur
contact emailthibaut.douche@pasteur.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013219
PRIDE project URI
Repository Record List
[ + ]