PXD013019

PXD013019 is an original dataset announced via ProteomeXchange.

Title	A chemical strategy for protease substrate profiling
Description	Here, we introduce a simple method, termed CHOPS, for the discovery of protease substrates. CHOPS exploits a 2-pyridinecarboxaldehyde (2PCA)-biotin probe, which selectively biotinylates protein N-termini except those with proline in the second position. CHOPS can, in theory, discover substrates for any protease, but is particularly well-suited to discover canonical DPP substrates, as cleaved but not intact DPP substrates can be identified by gel electrophoresis or mass spectrometry. This work delineates a practical technology for identifying protease substrates, which we anticipate will complement available “N-terminomic” approaches.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:11:18.006.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Griswold
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-08 08:08:18	ID requested
1	2019-04-23 05:00:33	announced
⏵ 2	2024-10-22 04:11:22	announced	2024-10-22: Updated project metadata.

10.1016/j.chembiol.2019.03.007;

Griswold AR, Cifani P, Rao SD, Axelrod AJ, Miele MM, Hendrickson RC, Kentsis A, Bachovchin DA, A Chemical Strategy for Protease Substrate Profiling. Cell Chem Biol, 26(6):901-907.e6(2019) [pubmed]

submitter keyword: CHOPS,SILAC, N-terminomics

Daniel A. Bachovchin
contact affiliation	Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
contact email	bachovcd@mskcc.org
lab head
Andrew Griswold
contact affiliation	Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, New York, USA.
contact email	ang2055@med.cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD013019

PRIDE project URI

• PRIDE
  1. PXD013019
     1. Label: PRIDE project
     2. Name: A chemical strategy for protease substrate profiling