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PXD012848 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIsobaric tags for relative and absolute quantification based on quantitative proteomic analysis on inhibitor of apoptosis protein-like protein-2 knocked-down MCF-7 breast cancer cells
DescriptionAlthough the inhibitor of apoptosis protein-like protein-2 (ILP-2) is regarded as a novel growth enhancer for breast cancer, its mechanism on breast cancer cell growth remains elusive and requires further study. In this study, isobaric tags were applied for relative and absolute quantification (iTRAQ) analysis on two groups of MCF-7 (Michigan Cancer Foundation-7) breast cancer cells, namely, siRNA-5 group (knocked down, KD group) and negative control (NC) group, to analyse the protein expression profiles correlated to ILP-2 during breast cancer cell growth. Western blot was applied to verify the iTRAQ data. The results indicated that a total of 4065 proteins were identified in breast cancer cells, and 241 of these proteins are differentially expressed (fold change ≥ 1.20 or ≤ 0.83 and P<0.05). A total of 156 up- and 85 down-regulated proteins are found in siRNA-5 group versus NC group. These differentially expressed proteins are principally correlated to the ECM (extracellular matrix) -receptor interaction, and the proteins from the top 10 biological processes are correlated to signal transduction, regulation of cell proliferation and immune system processes. The expression of AGA (N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase), MT1E (metallothionein-1E) and TDO2 (tryptophan 2,3-dioxygenase) increases when the protein expression of ILP-2 is knocked down. These results suggested that ILP-2 has an important role in breast cancer cell growth via protein interaction and provided comprehensive insights into biochemical pathways and regulation networks correlated to ILP-2.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMingJun Xiang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-24 23:30:35ID requested
12021-12-28 19:12:00announced
22023-03-01 23:02:59announced2023-03-02: Update publication information.
Publication List
Xiang S, Zhu L, Zhang Z, Wang S, Cui R, Xiang M, 2 on breast cancer cell proliferation. Mol Med Rep, 25(3):(2022) [pubmed]
Keyword List
submitter keyword: breast cancer, inhibitor of apoptosis protein-like protein-2, isobaric tags for relative and absolute quantification, proteome, MCF-7
Contact List
MingJun Xiang
contact affiliationJishou University
contact emailxmj688@163.com
lab head
MingJun Xiang
contact affiliationJishou University
contact emailxmj688@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI