PXD012737

PXD012737 is an original dataset announced via ProteomeXchange.

Title	Tools for flesh quality and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus labrax) fillets
Description	Fresh fish are highly perishable food products and their short shelf-life limits their commercial exploitation, leads to waste and has a negative impact on aquaculture sustainability. New non-thermal food processing methods, such as High pressure (HP), are being investigated to prolong shelf-life while assuring high food quality. We applied several tools to evaluate the impacts of HP processing on European sea bass (Dicentrarchus labrax) fillets quality and shelf life. The data here presented includes visual and physical measurements of flesh quality and the microbiome and proteome profiles of control and HP-processed sea bass fillets (600MPa, 25ºC, 5min), after isothermal storage (2°C) for different periods ranging from 1 to 67 days. Color (L-, a- and b- values) change and texture (hardness, cohesiveness and adhesiveness) parameters were obtained by using appropriate colorimeter and texture analyser, respectively, during refrigerated storage. Bacterial diversity was analysed by Illumina high-throughput sequencing of the 16S rRNA gene in five pooled DNAs from control or HP-processed fillets after 1, 11 or 67 days and the raw reads were deposited in the NCBI-SRA database with accession number PRJNA517618. In addition, high-throughput sequencing of the internal transcribed spacer (ITS) region targeting yeast and moulds was run for control or HP-processed fillets at the end of storage (11 or 67 days, respectively), being deposited under SRA accession PRJNA517779. Quantitative label-free proteomics profiles were analysed by SWATH-MS (Sequential Windowed data independent Acquisition of the Total High-resolution-Mass Spectra) in myofibrillar or sarcoplasmic enriched protein extracts pooled for control or HP-processed filets after short (1d) or long-term (11-67 days) storage. These data support the findings reported in “High pressure processing of European sea bass (Dicentrarchus labrax) fillets and tools for flesh quality and shelf life monitoring” (Tsironi et al. 2019).
HostingRepository	PRIDE
AnnounceDate	2019-11-06
AnnouncementXML	Submission_2019-11-06_01:52:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cátia Santa
SpeciesList	scientific name: Dicentrarchus labrax; NCBI TaxID: 13489;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-15 08:35:54	ID requested
⏵ 1	2019-11-06 01:52:52	announced

Anjos L, Pinto PIS, Tsironi T, Dimopoulos G, Santos S, Santa C, Manadas B, Canario A, Taoukis P, Power DM, ) fillets. Data Brief, 26():104451(2019) [pubmed]

submitter keyword: European sea bass

fillet

high pressure

metagenomics

proteomics

SWATH

Bruno Manadas
contact affiliation	Center for Neuroscience and Cell Biology
contact email	bmanadas@gmail.com
lab head
Cátia Santa
contact affiliation	Center for Neuroscience and Cell Biology
contact email	catiajmsanta@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012737
     1. Label: PRIDE project
     2. Name: Tools for flesh quality and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus labrax) fillets