Nematostella vectensis is a venomous organism with a complex life cycle. This dataset is to complement the dataset PXD008218 where proteoms of several Nematostella life stages (unfertilised eggs, planulae, primary polyp, adult male and female) were analyzed to compare toxins abbunances. Here we re-ran unfertilized eggs LC-MSMS (4 replicates) separately from other life stages to eliminate cross-contamination with abbundant toxins expressed at adult stage. To control for comparability to the older PXD008218 dataset we also re-ran adult female LC-MSMS (1 replicate). Same samples as inPXD008218 were used.