These data accompany our publication "RIT1 oncoproteins escape LZTR1-mediated proteolysis" (Castel P., et al. Science, 2019). Experiment 1: Identification of LZTR1 as an interacting partner of RIT1 Q Exactive Plus P20161208-02 FLAG GFP P20161208-03 FLAG RIT1 LTQ Orbitrap Velos V20170407-01 GST empty vector V20170407-03 GST RIT1 V20170407-06 HA empty vector V20170407-07 HA RIT1 Experiment 2: Identification of K135 and K187 ubiquitination sites in RIT1 Q Exactive Plus P20180408-01 FLAG-RIT1 / empty vector / empty vector (replicate 1) P20180408-02 FLAG-RIT1 / LZTR1 / empty vector (replicate 1) P20180408-03 FLAG-RIT1 / empty vector / TUBE (replicate 1) P20180408-04 FLAG-RIT1 / LZTR1 / TUBE (replicate 1) P20180408-06 GST-RIT1 / empty vector / empty vector (replicate 1) P20180408-07 GST-RIT1 / LZTR1 / empty vector (replicate 1) P20180408-08 GST-RIT1 / empty vector / TUBE (replicate 1) P20180408-09 GST-RIT1 / LZTR1 / TUBE (replicate 1) P20180408-11 GST-RIT1 / empty vector / empty vector (replicate 2) P20180408-12 GST-RIT1 / LZTR1 / empty vector (replicate 2) P20180408-13 GST-RIT1 / empty vector / TUBE (replicate 2) P20180408-14 GST-RIT1 / LZTR1 / TUBE (replicate 2) P20180408-16 FLAG-RIT1 / empty vector / empty vector (replicate 2) P20180408-17 FLAG-RIT1 / LZTR1 / empty vector (replicate 2) P20180408-18 FLAG-RIT1 / empty vector / TUBE (replicate 2) P20180408-32 FLAG-RIT1 / LZTR1 / TUBE (replicate 2)