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PXD012370 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA folded conformation of MukBEF and Cohesin
DescriptionPleasStructural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but the underlying conformational changes are largely unclear. Using structural biology, mass spectrometry and cross-linking, we investigated the architecture of two evolutionarily distant SMC-kleisin complexes: proteobacterial MukBEF and eukaryotic cohesin. We show that both contain a dynamic coiled-coil discontinuity, the elbow, near the middle of their arms that permits a folded conformation. Bending at the elbow brings into proximity the hinge dimerization domain and the head/kleisin module, situated at opposite ends of the arms. Our findings favor SMC activity models that include a large conformational change in the arms, such as a relative movement between DNA binding sites during DNA loading and translocatione provide an overall description of your study, think something similar in scope to the manuscript abstract
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrancis O'Reilly
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-15 23:42:22ID requested
12019-03-01 00:15:15announced
22019-05-27 01:17:17announcedUpdated publication reference for PubMed record(s): 30833788.
Publication List
B, ü, rmann F, Lee BG, Than T, Sinn L, O'Reilly FJ, Yatskevich S, Rappsilber J, Hu B, Nasmyth K, L, ö, we J, A folded conformation of MukBEF and cohesin. Nat Struct Mol Biol, 26(3):227-236(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: crosslinking, MukBEF, cohesin, condensin, kleisin, Structural Maintenance of Chromosomes, SMC proteins, DNA translocation, loop extrusion
Contact List
Juri Rappsilber
contact affiliationBioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany; Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK.
contact emailjuri.rappsilber@ed.ac.uk
lab head
Francis O'Reilly
contact affiliationTU Berlin
contact emailoreilly@tu-berlin.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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