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PXD012263 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunctional proteomic atlas of HIV-infection in primary human CD4+ T cells
DescriptionViruses manipulate host cells to enhance their replication, and the identification of host factors targeted by viruses has led to key insights in both viral pathogenesis and cellular physiology. We previously described global changes in cellular protein levels during human immunodeficiency virus (HIV) infection using transformed CEM-T4 T cells as a model. In this study, we develop an HIV reporter virus displaying a streptavidin-binding affinity tag at the surface of infected cells, allowing facile one-step selection with streptavidin-conjugated magnetic beads. We use this system to obtain pure populations of HIV-infected primary human CD4+ T cells for detailed proteomic analysis, including quantitation of >9,000 proteins across 4 different donors, and temporal profiling during T cell activation. Remarkably, amongst 650 cellular proteins significantly perturbed during HIV infection of primary T cells (q<0.05), almost 50% are regulated directly or indirectly by the viral accessory proteins Vpr, Vif, Nef and Vpu. The remainder have not been previously characterised, but include novel Vif-dependent targets FMR1 and DPH7, and 192 targets not identified and/or regulated in T cell lines, such as AIRD5A and PTPN22. We therefore provide a high-coverage functional proteomic atlas of HIV infection, and a mechanistic account of HIV-dependent changes in its natural target cell.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJames Williamson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListAcetylhypusine; TMT6plex; Oxidation; MappingA; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-08 00:28:51ID requested
12019-03-08 01:37:57announced
22019-05-27 01:16:56announcedUpdated publication reference for PubMed record(s): 30857592.
Publication List
Naamati A, Williamson JC, Greenwood EJ, Marelli S, Lehner PJ, Matheson NJ, Functional proteomic atlas of HIV infection in primary human CD4+ T cells. Elife, 8():(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Proteomics, HIV, Primary T-Cells
Contact List
Nicholas J Matheson
contact affiliationDepartment of Medicine, University of Cambridge, Cambridge Biomedical Campus, Cambridge, CB2 0XY, UK
contact emailnjm25@cam.ac.uk
lab head
James Williamson
contact affiliationUniversity of Cambridge
contact emailjcw76@cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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