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PXD012158 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titleproteomics and phosphoprotemics analysis of Ndufs4 KO and wild-type mouse brain upon rapamycin treatment
Description+ 2 experiments: - effect of rapamycin in Ndufs4 KO mouse --> 3 groups: WT, KO and KR (KO+RP) - effect of rapamycin in wild-type mouse --> 2 groups: WT and WR (WT+RP) + samples from whole brains homogenized in liquid nitrogen + proteins digested with trypsin. No fractionation + phosphopeptides enriched with IMAC beads + instruments: Orbitrap Q Exactive (for proteome), Orbitrap LTQ Velos (for phosphoproteome) + 90 minutes gradient runs + search and quantification with MaxQuant
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMiguel Martin Perez
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
InstrumentQ Exactive; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-21 07:15:23ID requested
12020-07-15 22:26:29announced
Publication List
no publication
Keyword List
submitter keyword: brain, mouse, mitochondria, leigh syndrome, rapamycin, signalling, phosphorylation, respiratory complex I, ndufs4, NADH ubiquinone, proteome, phosphoproteome, PKC, mTOR, respiratory chain, inflammation, neurodegeneration
Contact List
Judit Villen
contact affiliationUniversity of Washington
contact emailjvillen@uw.edu
lab head
Miguel Martin Perez
contact affiliationUniversity of Washington
contact emailmmarper@uw.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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