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PXD012094 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS analysis of an intact synthetic microRNA
DescriptionThe field of epitranscriptomics is growing in importance, with chemical modification of RNA being associated with a wide variety of biological phenomena. Mass spectrometry (MS) enables the identification of modified RNA residues within their sequence contexts, by using analogous approaches to shotgun proteomics. We have developed a free and open-source database search engine for RNA MS data, called NucleicAcidSearchEngine (NASE), as part of the OpenMS software framework. NASE allows the reliable identification of (modified) RNA sequences from LC-MS/MS data in a high-throughput fashion. For this validation dataset, oligonucleotides with the sequence of mature Drosophila let-7 microRNA, 21 nt in length, were produced synthetically in unmodified and modified (2’-O-methylated at the 3’ uridine) forms. We characterised a 1:1 mixture of both forms of this RNA.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHendrik Weisser
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonomethylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-18 08:15:27ID requested
12018-12-31 03:18:49announced
22019-01-11 03:53:52announcedUpdated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: RNA, miRNA, let-7, epitranscriptomics
Contact List
Byron Andrews
contact affiliationSTORM Therapeutics Limited Cambridge United Kingdom
contact emailbyron.andrews@stormtherapeutics.com
lab head
Hendrik Weisser
contact affiliationSTORM Therapeutics Limited
contact emailhendrik.weisser@stormtherapeutics.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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