E. coli BW25113 growth in a nutrient-rich medium supplemented with 20 proteinogenic amino acid medium was studied and compared to growth in minimal medium. Proteome data from these experiments revealed significant differences in amino acid synthesis and transport proteins that were reduced in the nutrient-rich meidum. Changes in energy production and conversion proteins were also observed as in nutrient-rich conditions pyruvate dehydrogenase complex and acetate producing proteins increased, while the TCA cycle proteins decreased.