Updated project metadata. In field conditions, tea plants are often exposed to drought stress, which has profound effects on the growth and development of tea plants. However, most studies on tea plants in response to drought stress focused on single gene or protein expression, and transcriptome or proteome profiles, the impact of drought stress on ubiquitination in proteins remains unearthed. We performed a global profile of ubiquitinated (Kub) proteins in tea leaves under drought stress. In total, 1,409 lysine Kub sites in 781 proteins were identified, of which 14 sites in 12 proteins were up-regulated and 123 sites in 91 proteins were down-regulated compared with drought and control. Furthermore, we analyzed the Kub proteins related to ubiquitin-mediated proteolysis, catechins biosynthesis, and carbohydrate and amino acid metabolism in tea leaves under drought stress. The results indicated that many Kub proteins involved in ubiquitin-mediated proteolysis played important roles in protein degradation. Several Kub proteins related to catechins biosynthesis were positively correlated with each other because of their co-expression and co-localization. Our study preliminarily revealed the global profiling of Kub proteins in metabolic pathways and provided an important resource for further study on the functions of Kub proteins in tea plants under drought stress.