<<< Full experiment listing


PXD011610 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAmmonium resupply-triggered response mechanism of lysine crotonylome in tea leaves (Camellia sinensis L.)
DescriptionBackground: Lysine crotonylation (Kcr), as a novel evolutionarily conserved type of PTM, is ubiquitous and essential in cell biology. However, its functions in tea plant, an important beverage crop, are largely unknown. Our study firstly attempted to describe Kcr proteins in tea leaves under NH4+ deficiency/resupply, and provided significant insights into exploring the physiological role of Kcr in plants for N utilization. Results: We performed the global analysis of crotonylome in tea plants under NH4+ deficiency/resupply using high-resolution LC-MS/MS coupled with highly sensitive immune-antibody. A total of 2288 kcr sites on 971 proteins were identified, of which contained in 15 types of Kcr motifs. Most of Kcr proteins were located in chloroplast and cytoplasm. 120 and 151 Kcr proteins were significantly changed at 3 hours and 3days of NH4+ resupply, respectively. Bioinformatics analysis showed that differentially expressed Kcr proteins participated in diverse biological processes such as photosynthesis, carbon fixation and amino acid metabolism, suggesting Kcr plays important roles in these processes. Interestingly, a large number of enzymes were crotonylated, and the activity and Kcr level of these enzymes changed significantly after NH4+ resupply, indicating a potential function of Kcr in the regulation of enzyme activities. Moreover, the protein-protein interaction analysis revealed that the diverse interactions of identified Kcr proteins mainly involved in photosynthesis, carbon fixation, amino acid metabolism and ribosome. Conclusions: The results suggested that lysine crotonylated proteins might play regulating roles in metabolic process in tea leaves under NH4+ deficiency/resupply. The critical regulatory roles mainly involved in diverse aspects of primary metabolic processes, especially in photosynthesis, carbon fixation and amino acid metabolism. The provided data may serve as important resources for exploring the physiological, biochemical, and genetic role of lysine crotonylation in tea plants.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterlihuan Qin
SpeciesList scientific name: Camellia sinensis; NCBI TaxID: 4442;
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-07 08:13:44ID requested
12019-05-09 00:27:17announced
Publication List
Sun J, Qiu C, Qian W, Wang Y, Sun L, Li Y, Ding Z, Ammonium triggered the response mechanism of lysine crotonylome in tea plants. BMC Genomics, 20(1):340(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Lysine crotonylation, Crotonylome, Camellia sinensis L., Photosynthesis, Carbon fixation, Amino acid metabolism, Enzymatic activity
Contact List
Zhaotang Ding
contact affiliationTea Research Institute, Qingdao Agricultural University, Qingdao, Shandong 266109, China
contact emaildzttea@163.com
lab head
lihuan Qin
contact affiliationPTM Biolabs Inc.
contact emaillihuan_qin@ptm-biolab.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD011610
PRIDE project URI
Repository Record List
[ + ]