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PXD011045 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA conserved atypical MFS family member orchestrates a subset of O-glycosylation to facilitate macrophage dissemination and tissue invasion
DescriptionAberrant display of the truncated core1 O-glycan T-antigen is a common feature of human cancer cells that correlates with metastasis. Here we show that T-antigen in Drosophila melanogaster macrophages is involved in their developmentally programmed tissue invasion. Higher macrophage T-antigen levels require an atypical major facilitator superfamily (MFS) member that we named Minerva which enables macrophage dissemination and invasion. We characterize for the first time the T and Tn glycoform O-glycoproteome of the Drosophila melanogaster embryo, and determine that Minerva increases the presence of T-antigen on protein pathways previously linked to cancer, most strongly on the protein sulfhydryl oxidase Qsox1 which we show is required for macrophage invasion. Minerva’s vertebrate ortholog, MFSD1, rescues the minerva mutant’s migration and T-antigen glycosylation defects. We thus identify a key conserved regulator that orchestrates O-glycosylation on a protein subset to activate a program governing migration steps important for both development and cancer metastasis
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSergey Vakhrushev
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonohydroxylated residue; Hex-HexNAc; N-acetylaminohexosylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-10 03:48:20ID requested
12019-04-01 00:57:55announced
Publication List
Valoskova K, Biebl J, Roblek M, Emtenani S, Gyoergy A, Misova M, Ratheesh A, Reis-Rodrigues P, Shkarina K, Larsen ISB, Vakhrushev SY, Clausen H, Siekhaus DE, A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion. Elife, 8():(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: embryo, migration, major facilitator superfamily, O-glycosylation, invasion, dissemination, metastasis, glycoproteomics, ETD, drosophila melanogaster
Contact List
Daria E. Siekhaus
contact affiliationInstitute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria
contact emaildaria.siekhaus@ist.ac.at
lab head
Sergey Vakhrushev
contact affiliationDepartment of Cellular and Molecular Medicine
contact emailseva@sund.ku.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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