PXD010859 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Precisely measured protein lifetimes in the mouse |
Description | The turnover of brain proteins is critical for organism survival, and its perturbations are strongly linked to pathology. Nevertheless, precise protein lifetimes have been difficult to obtain in vivo. They are readily measured in vitro by feeding cells with isotopically-labeled amino acids, followed by mass spectrometry analyses. In vivo proteins are generated from at least two sources: labeled amino acids from the diet, and non-labeled amino acids from the degradation of pre-existing proteins. This renders measurements difficult. We solved this problem rigorously by developing a novel workflow that combines mouse in vivo isotopic labeling with mass spectrometry and mathematical modeling. We also established several independent approaches to test and validate the results. This enabled us to measure, for the first time, the accurate lifetimes of ~3500 brain proteins. The high precision of our data provided a large set of biologically-significant observations, including pathway-, organelle-, organ-, or cell-specific effects, along with a comprehensive catalogue of stable proteins, including novel extremely long-lived proteins (ELLPs). |
HostingRepository | PRIDE |
AnnounceDate | 2018-12-04 |
AnnouncementXML | Submission_2018-12-04_04:07:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sunit Mandad |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-24 02:00:45 | ID requested | |
1 | 2018-11-21 02:44:38 | announced | |
⏵ 2 | 2018-12-04 04:07:58 | announced | Updated project metadata. |
Publication List
Fornasiero EF, Mandad S, Wildhagen H, Alevra M, Rammner B, Keihani S, Opazo F, Urban I, Ischebeck T, Sakib MS, Fard MK, Kirli K, Centeno TP, Vidal RO, Rahman RU, Benito E, Fischer A, Dennerlein S, Rehling P, Feussner I, Bonn S, Simons M, Urlaub H, Rizzoli SO, Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions. Nat Commun, 9(1):4230(2018) [pubmed] |
Keyword List
ProteomeXchange project tag: Human Brain Proteome Project (HUPO_HBPP) (B/D-HPP) |
curator keyword: Biological |
submitter keyword: Protein lifetimes, metabolic labeling, brain turnover, ELLPs, neuron, glia, cortex, cerebellum |
Contact List
Silvio O. Rizzoli |
contact affiliation | Department of Neuro- and Sensory Physiology University Medical Center Göttingen Humboldtallee 23 37073 Göttingen |
contact email | srizzol@gwdg.de |
lab head | |
Sunit Mandad |
contact affiliation | University Medical Centre Göttingen, Georg-August-Universität |
contact email | smandad@gwdg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010859
- Label: PRIDE project
- Name: Precisely measured protein lifetimes in the mouse