UMR106 cells were untreated or treated with 5 mM Pi or 100 ng/ml FGF2 for 15 min. The lysates were digested with trypsin, and tyrosine-phosphorylated peptides were enriched by anti-phosphotyrosine antibody and subjected to LC-MS/MS analysis. Raw data were directly analyzed against the SwissProt database restricted to Rattus using Proteome Discoverer version 2.2 for identification and label-free precursor ion quantification.