293T cells were infected with HIV-1 pseudovirions, and 0, 4 and 12 hours post-infection, the cell lysates were collected. Label-free proteomics was applied to examine the protein-level changes. Duplicate samples for six time points were collected (0, 4, and 12 hours post-transduction, virus- and mock transduced samples) and analyzed in duplicates, allowing for the measurement of two technical and two biological replicates for each time point. The results were used for the analysis of protein networks implementing both the qualitative and quantitative information.