<<< Full experiment listing


PXD010324 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of Arabidopsis mitochondrial ribosome proteome
DescriptionIn order to identify the comprehensive composition of Arabidopsis mitochondrial ribosome proteins, a strategy based on complementary approaches was used. Classical biochemical purification of ribosomes was combined with the immunoprecipitation of mitoribosomes using a specific Arabidopsis mitoribosome protein as a bait and quantitative proteomics. The immuno-purifications (IP) of the mitoribosome were performed on purified mitochondria from rPPR1-HA plant line (flowers as starting material). IPs were performed performed in different conditions (i.e. with 100, 400 or 600 mM KCl at the IP washing step or 800mM all along the IP). For each condition, experiments were performed in triplicates and IP proteins were identified by quantitative nano LC-ESI-MS/MS. Classic biochemical purification of mitochondrial monosomes (i.e. free ribosomes) was also performed. Starting from purified wild-type Arabidopsis mitochondria (cell culture as starting material), ribosomes were separated on high resolution 10-30% continuous sucrose gradients and mitoribosome containing fractions were analyzed by quantitative nano LC-ESI-MS/MS.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLauriane Kuhn
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-06 01:16:31ID requested
12018-12-14 03:15:21announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Arabidopsis, mitochondria, ribosome, nanoLC-ESI-MS/MS
Contact List
Florent Waltz
contact affiliationInstitut de biologie de moléculaire des plantes, UPR2357 du CNRS, Université de Strasbourg, 12 rue du général Zimmer, F-67084 Strasbourg, France
contact emailwaltz.florent@gmail.com
lab head
Lauriane Kuhn
contact affiliationCNRS, IBMC, FRC1589
contact emaill.kuhn@ibmc-cnrs.unistra.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD010324
PRIDE project URI
Repository Record List
[ + ]