Updated FTP location. Investigating the profile of normal urinary peptidome, and the peptidases involved in its generation is extremely important as it reflects the human normal physiology, and the incidence of any change can be used as an indicator for pathological processes occurred in vivo. Thus, in this study, we interested in the characterization of normal urinary peptidome as well as investigating the different catalytic enzymes which involved in urinary native peptide generation.