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PXD009945 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHigh definition analysis of protein stability during cytomegalovirus infection informs on cellular restriction
DescriptionHuman cytomegalovirus (HCMV) is an important pathogen and a paradigm of viral immune evasion. Here, we describe a multiplexed approach to discover proteins with novel innate immune functions on the basis of their active proteasomal or lysosomal degradation during the early phase of HCMV infection. Using three orthogonal proteomic/transcriptomic screens to quantify protein degradation, we identified 35 proteins with high confidence that were enriched in known antiviral restriction factors. A final screen facilitated global analysis of the mechanism of viral immune-modulation by predicting which viral genes target >250 human proteins. Helicase-like Transcription Factor (HLTF), a DNA helicase important in error-free post-replication DNA repair, was rapidly degraded during infection and potently inhibited early viral gene expression. HCMV UL145, a protein of previously unknown function, recruits the Cullin 4 E3 ligase complex to degrade HLTF. Our approach and data will enable further identifications of innate pathways targeted by HCMV and other viruses.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Weekes
SpeciesList scientific name: Cytomegalovirus; NCBI TaxID: 10358; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-29 01:27:26ID requested
12018-08-16 08:17:41announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: quantitative proteomics, tandem mass tag, pulsed SILAC, host-pathogen interaction, immune evasion, innate immunity, restriction factor, proteasome, lysosome, protein degradation
Contact List
Michael Weekes
contact affiliationCambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK
contact emailMpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliationUniversity of Cambridge
contact emailmpw1001@cam.ac.uk
dataset submitter
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Dataset FTP location
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