This submission contains the mass spectrometry files for the manuscript by Chapman and colleagues that characterize KRI-1 in C. elegans by affinity purification experiments of KRI-1 fused to GFP. Controls used for this experiment include ACT-5 fused to GFP and N2 worms. This dataset consists of 18 raw MS files and associated with peak lists (mzML) and mass spectrometry results (mzid) in standard formats, acquired on a Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap operated in DDA mode. Samples were generated by Eric Chapman and affinity purification, mass spectrometric acquisition and analysis was performed by Christopher Go. Raw files are deposited here in MassIVE. Brent Derry is the corresponding author of the manuscript (brent.derry@sickkids.ca); Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca).