We collected a total of 9.8 million mass spectra generated in the laboratory from the proteomics analyses of different silkworm tissues, including the posterior silk gland (PSG) 30, middle silk gland 31, ovary and testis 32, head 33, brain, prothoracic glands, subesophageal ganglion 34, hemolymph 35, fat body 36 and embryo 37,38 of domestic silkworm, and the posterior silk gland of wild silkworm 39.