PXD009249

PXD009249 is an original dataset announced via ProteomeXchange.

Title	ATO and Gossypol for GSC treatment
Description	Glioblastoma is one of the deadliest malignancies worldwide and is virtually incurable due to its highly infiltrative growth and limited sensitivity to conventional treatment by radiochemotherapy. It is hypothesized that a small population of cells with a stem-like phenotype is the major culprit of tumor recurrence. These cells are characterized by an enhanced DNA repair capacity and expression of stemness marker genes, and elimination of this population might delay or even stop tumor recurrence following radiochemotherapy. The aim of this study was to analyze whether interference with the Hedgehog signaling (Hh) pathway or combined Hh/Notch blockade can efficiently target these cancer stem cells and sensitize them to chemotherapeutic drugs. Using tumor sphere lines and primary glioma cells we demonstrate that the Hh pathway inhibitor GANT61 (GANT) and the Hh/Notch inhibitor arsenic trioxide (ATO) are capable to synergistically decrease proliferation and induce cell death in combination with the natural cotton derived anticancer agent (-)-Gossypol (Gos). In contrast to GANT in combination with Gos, the ATO/Gos combination also strongly prevented sphere formation and recovery. These synergistic effects were associated with major proteomic changes indicating decreased cell movement, distortion of cell cycle and DNA repair, as well as markedly reduced stemness. Collectively, our data show that ATO and Gos, two drugs that are safe for use in humans, represent a promising targeted therapy approach for the synergistic and specific elimination of glioma stem-like cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:49.974.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Bonn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-19 01:14:05	ID requested
1	2019-03-12 20:42:07	announced
2	2019-03-20 06:51:07	announced	Updated publication reference for PubMed record(s): 30871073.
⏵ 3	2024-10-22 04:43:53	announced	2024-10-22: Updated project metadata.

10.3390/cancers11030350;

Linder B, Wehle A, Hehlgans S, Bonn F, Dikic I, R, ö, del F, Seifert V, K, ö, gel D, Arsenic Trioxide and (-)-Gossypol Synergistically Target Glioma Stem-Like Cells via Inhibition of Hedgehog and Notch Signaling. Cancers (Basel), 11(3):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Chemotherapy,Gliablastoma

Donat Koegel
contact affiliation	University Hospital, Experimental Neurosurgery
contact email	koegel@em.uni-frankfurt.de
lab head
Florian Bonn
contact affiliation	University of Frankfurt
contact email	flobonn@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD009249

PRIDE project URI

• PRIDE
  1. PXD009249
     1. Label: PRIDE project
     2. Name: ATO and Gossypol for GSC treatment