Updated project metadata. The study of peptide secretion, and identifying its control requires the use of ELISAs for measuring their levels pre and post stimulation. In place of ELISA's an LC-MS methdology was developed first to identify the correct peptides generated by the neurons, which could then be used to measure the peptide products by targetted LC-MS analysis. The Extraction was also performed on human hypothalamic tissue obtained from a tissue bank. Samples were analysed by nano LC-MS, and searched using Peaks 8.5.