PXD008498

PXD008498 is an original dataset announced via ProteomeXchange.

Title	Cisplatin induces the rearrangement of RBPs on CDKN1B/p27 mRNA
Description	Cisplatin (CP) is a chemotherapeutic drug that is used to cure different types of cancer. CP induces DNA damage and leads to cell cycle arrest. The cyclin-dependent kinase inhibitor 1B (CDKN1B), also termed p27, plays an important role in the drug response ; and increased levels of p27 correlated with CP resistance. In HEK293 cells, we observed that p27 mRNAs levels increased whereas protein level drastically decreased in cells treated with CP; suggesting post-transcriptional regulatory events. To further understand the underlying mechanisms, we applied a biochemical approach combined with mass-spectrometry to systematically identify the RNA-binding proteins (RBPs) that are bound to the 3’UTR of p27 mRNAs in CP-treated versus non-treated cells in vivo. We found that 24 proteins, most of them known RBPs such HuR, hNRNPD, changed their association with p27 mRNA upon CP treatement. Furthermore, knock-down of a subset of the identified RBPs led to the inhibition of the CP-induced increase of p27 mRNA levels. In conclusion, these results highlight substantial rearrangement between RBPs and p27 mRNA upon CP treatment and corroborate the importance of post-transcriptional control in cellular drug response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:42.305.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valentina Iadevaia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-19 04:52:28	ID requested
1	2019-09-24 07:17:24	announced
⏵ 2	2024-10-22 04:42:48	announced	2024-10-22: Updated project metadata.

10.1080/15476286.2019.1662268;

Iadevaia V, Wouters MD, Kanitz A, Matia-Gonz, á, lez AM, Laing EE, Gerber AP, 3'UTRs in cisplatin treated cells. RNA Biol, 17(1):33-46(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Tobramaycin, RNA aptamer,RBPs, CDKN1B/p27

Valentina Iadevaia
contact affiliation	University of Surrey
contact email	v.iadevaia@surrey.ac.uk
lab head
Valentina Iadevaia
contact affiliation	University of Surrey
contact email	v.iadevaia@surrey.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD008498

PRIDE project URI

• PRIDE
  1. PXD008498
     1. Label: PRIDE project
     2. Name: Cisplatin induces the rearrangement of RBPs on CDKN1B/p27 mRNA