Updated project metadata. Within the scope of a technical note we tested our MetaProteomeAnalyzer Portable software workflow on experimental data sets from samples with known composition. For generating a benchmarking data set, the bacterial strains (5BCT) Bacillus subtilis, Escherichia coli, Pseudomonas fluorescens, Micrococcus luteus and Desulfovibrio vulgaris were mixed with a protein ratio of 1:1:1:1:1.