PXD007570

PXD007570 is an original dataset announced via ProteomeXchange.

Title	Mapping Protein Targets of Bioactive Small Molecules using Lipid-Based Chemical Proteomics
Description	Lipids play critical roles in cell biology, often through direct interactions with proteins. We recently described the use of photoreactive lipid probes combined with quantitative mass spectrometry to globally map lipid-protein interactions, and the effects of drugs on these interactions, in cells. Here, we investigate the broader potential of lipid-based chemical proteomic probes for determining the cellular targets of biologically active small molecules, including natural product derivatives and repurposed drugs of ill-defined mechanisms. We identify the prostaglandin-regulatory enzyme PTGR2 as a target of the anti-diabetic hops derivative KDT501 and show that miconazole—an anti-fungal drug that attenuates disease severity in preclinical models of multiple sclerosis—inhibits SGPL1, an enzyme that degrades the signaling lipid sphingosine-1-phosphate, drug analogues of which are used to treat multiple sclerosis in humans. Our findings highlight the versatility of lipid-based chemical proteomics probes for mapping small molecule-protein interactions in human cells to gain mechanistic understanding of bioactive compounds.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:02:24.488.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kenneth Lum
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-28 05:47:59	ID requested
1	2017-09-26 05:07:09	announced
⏵ 2	2024-10-22 04:02:31	announced	2024-10-22: Updated project metadata.

Lum KM, Sato Y, Beyer BA, Plaisted WC, Anglin JL, Lairson LL, Cravatt BF, Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol, 12(10):2671-2681(2017) [pubmed]

10.1021/acschembio.7b00581;

curator keyword: Biomedical

submitter keyword: HEK293T, Neuro2A, Orbitrap Velos, chemoproteomics, lipid probes

Benjamin Cravatt
contact affiliation	Department of Molecular Medicine The Skaggs Institute for Chemical Biology The Scripps Research Institute USA
contact email	cravatt@scripps.edu
lab head
Kenneth Lum
contact affiliation	The Scripps Research Institute
contact email	lummyk@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD007570

PRIDE project URI

• PRIDE
  1. PXD007570
     1. Label: PRIDE project
     2. Name: Mapping Protein Targets of Bioactive Small Molecules using Lipid-Based Chemical Proteomics