PXD007230 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling |
Description | Following endocytosis into the endosomal network, integral membrane proteins undergo sorting for lysosomal degradation or are retrieved and recycled back to the cell surface. Here we describe the discovery of an ancient and conserved multiprotein complex which orchestrates cargo retrieval and recycling and importantly, is biochemically and functionally distinct to the established retromer pathway. Composed of a heterotrimer of DSCR3, C16orf62 and VPS29, and bearing striking similarity with retromer, we have called this complex ‘retriever’. We establish that retriever associates with the cargo adaptor sorting nexin 17 (SNX17) and couples to CCC (CCDC93, CCDC22, COMMD) and WASH complexes to prevent lysosomal degradation and promote cell surface recycling of α5β1-integrin. Through quantitative proteomic analysis we identify over 120 cell surface proteins, including numerous integrins, signalling receptors and solute transporters, which require SNX17-retriever to maintain their surface levels. Our identification of retriever establishes a major endosomal retrieval and recycling pathway. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-15 |
AnnouncementXML | Submission_2018-01-15_00:06:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kerrie McNally |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 6x(13)C labeled residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-09 05:57:27 | ID requested | |
⏵ 1 | 2018-01-15 00:06:18 | announced | |
Publication List
McNally KE, Faulkner R, Steinberg F, Gallon M, Ghai R, Pim D, Langton P, Pearson N, Danson CM, N, ä, gele H, Morris LL, Singla A, Overlee BL, Heesom KJ, Sessions R, Banks L, Collins BM, Berger I, Billadeau DD, Burstein E, Cullen PJ, Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. Nat Cell Biol, 19(10):1214-1225(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: retriever, retromer, SNX17 |
Contact List
Peter J Cullen |
contact affiliation | University of Bristol, School of Biochemistry |
contact email | Pete.Cullen@bristol.ac.uk |
lab head | |
Kerrie McNally |
contact affiliation | University of Bristol |
contact email | km13610@bristol.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007230 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007230
- Label: PRIDE project
- Name: Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling