PXD007149

PXD007149 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	4-Oxo-2-nonenal Adductions in Cells
Description	4-Oxo-2-nonenal (ONE) derived from lipid peroxidation modifies nucleophiles and transduces redox signaling by its reactions with proteins. However, the molecular interactions between ONE and complex proteomes and their dynamics in situ remain largely unknown. Here we de-scribe a quantitative chemoproteomic analysis of protein adduction by ONE in cells, in which the cellular target profile of ONE is mimicked by its alkynyl surrogate. The analysis reveals four types of ONE-derived modifications in cells, including ketoamide and Schiff-base adducts to lysine, Michael adducts to cysteine, and a novel pyrrole adducts to cysteine. ONE-derived adducts co-localize and crosstalk with many histone marks and redox sensitive sites. All four types of modifications derived from ONE can be reversed site-specifically in cells. In summary, our study provides much-needed mechanistic insights into the cellular signaling and potential toxicities associated with this important lipid derived electrophile.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:53.961.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007149
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jing Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-28 07:32:30	ID requested
1	2017-08-25 00:57:48	announced
⏵ 2	2024-10-22 04:40:00	announced	2024-10-22: Updated project metadata.

Publication List

Sun R, Fu L, Liu K, Tian C, Yang Y, Tallman KA, Porter NA, Liebler DC, Yang J, Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells. Mol Cell Proteomics, 16(10):1789-1800(2017) [pubmed]
10.1074/mcp.ra117.000116;
10.6019/PXD007149;

Sun R, Fu L, Liu K, Tian C, Yang Y, Tallman KA, Porter NA, Liebler DC, Yang J, Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells. Mol Cell Proteomics, 16(10):1789-1800(2017) [pubmed]

10.1074/mcp.ra117.000116;

10.6019/PXD007149;

Keyword List

curator keyword: Biological
submitter keyword: 4-Oxo-2-nonenal

curator keyword: Biological

submitter keyword: 4-Oxo-2-nonenal

Contact List

Yang Jing
contact affiliation	National Center for Protein Science Beijing
contact email	yangjing54@hotmail.com
lab head
Jing Yang
contact affiliation	National Center for Protein Sciences, Beijing
contact email	yangjing54@hotmail.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD007149

PRIDE project URI

Repository Record List

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