Updated project metadata. Recombinant PDCoV spike glycoprotein was produced from Drosophile S2 cells for structural characterization by cryo electron microscopy and mass spectrometry. MS was used to assess the N-linked glycosylation profile of the purified material. We used a multiple protease digestion in combination with RP-LC-MS/MS, EThcD fragmentation on Orbitrap Fusion to characterize intact glycopeptides.