<<< Full experiment listing


PXD006598 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamic Acclimation to High Light in Arabidopsis thaliana Involves Widespread Reengineering of the Leaf Proteome
DescriptionTypically, when fully developed leaves of Arabidopsis thaliana are exposed to an increase in light intensity, they are able to increase their photosynthetic capacity in a process known as dynamic acclimation. Fully developed leaves of Arabidopsis thaliana were exposed to a fourfold increase in light intensity for 7 days to induce high light acclimation. This treatment was subjected to wild-type and a non-acclimating mutant lacking the gpt2 gene. The proteomic responses of the leaves were investigated using label-free mass spectrometry. A large reorganisation of the proteome was shown, with increases in the abundance of proteins of photosynthesis and carbon metabolism. Subtle differences were seen between the WT and gpt2 mutant: in the mutant, an increased stress response was seen, and some differences in the responses of metabolism. Proteomic responses generally correlated with physiological responses.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Miller
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-26 04:40:02ID requested
12017-07-20 04:36:25announced
22017-08-07 05:21:34announcedUpdated publication reference for PubMed record(s): 28775726.
Publication List
Miller MAE, O'Cualain R, Selley J, Knight D, Karim MF, Hubbard SJ, Johnson GN, Involves Widespread Reengineering of the Leaf Proteome. Front Plant Sci, 8():1239(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Arabidopsis, label free, proteomics, high light acclimation, dynamic acclimation
Contact List
Giles N Johnson
contact affiliationSchool of Earth and Environmental Sciences, University of Manchester
contact emailgiles.johnson@manchester.ac.uk
lab head
Matthew Miller
contact affiliationAgrimetrics
contact emailmatt.miller@agrimetrics.co.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD006598
PRIDE project URI
Repository Record List
[ + ]