We report the targeted analysis of the human dental pulp stroma and the odontoblast layer using the positional proteomics technique TAILS N-terminomics, which allowed us to capture both naturally blocked and unblocked protein N-termini, and to identify differences between e.g. the dental pulp proteomes of older and younger donors. Noteworthy, these differences were most pronounced in the odontoblast region. Note: This study is a follow-up on PXD002264.