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PXD006301 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExpression of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta
DescriptionPlants and algae have developed various light harvesting mechanisms for optimal delivery of excitation energy to the photosystems. The cryptophyte algae have evolved a novel soluble light-harvesting antenna utilizing phycobilin pigments to complement the membrane-intrinsic Chl a/c-binding LHC antenna. This new antenna consists of the plastid-encoded beta-subunit, a relict of the ancestral phycobilisome, and a novel nuclear-encoded -subunit unique to cryptophytes. Together, these proteins form the active tetramer, which consists of one alpha 1 and one alpha 2 and two beta susbunits. In all cryptophyte algae investigated so far, the alpha-subunits have duplicated and diversified into a large gene family. Although there is transcriptional evidence for expression of all these genes, the x-ray structures determined to date suggest that only two of the -subunit genes might be significantly expressed at protein level. Using proteomics, we show that phycoerythrin 545 (PE545) of Guillardia theta, the only cryptophyte with a sequenced genome, all 20 alpha-subunits are expressed when the algae grow under white light. Their relative expression levels depend on the intensity of the growth light, but there is no evidence for a specific light-dependent regulation of individual members of the alpha-subunit family under the growth conditions applied. Subunit GtcpeA10 seems to be a special member of the alpha-subunit family, because it consists of two similar N- and C-terminal domains, which likely are the result of a partial gene duplication.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterThomas Kieselbach
SpeciesList scientific name: Guillardia theta CCMP2712; NCBI TaxID: 905079;
ModificationListDeamidated; Oxidation; Carbamidomethyl
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-04-13 08:28:30ID requested
12017-06-20 04:27:30announced
Publication List
Kieselbach T, Cheregi O, Green BR, Funk C, Proteomic analysis of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta cultured under different light intensities. Photosynth Res, 135(1-3):149-163(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Plant, cryptophyte, Gulillarida theta, phycobiliprotein, LC_MS/MS, proteomics
Contact List
Thomas Kieselbach
contact affiliationUmea University, Department of Chemistry, 90187 Umea, Sweden
contact emailthomas.kieselbach@chem.umu.se
lab head
Thomas Kieselbach
contact affiliationBiochemistry
contact emailthomas.kieselbach@chem.umu.se
dataset submitter
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Dataset FTP location
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