Updated project metadata. This study aims to investigate changes in phospho-proteomic levels of mice brain. The focus of the study is the phospho-proteome because phoshorylation is one of the key post-translational modification, which regulates many biological processes. In particular we wanted to inspect the effects of two different kinds of diet (hyperglycemic diet and high-fat diet) compaired with a conventional chow diet. We have found interesting changes in the phosphorylation state of several proteins involved in nervous system development as well as vesicle trafficking and cytoskeleton remodelling.We hope that our study can contribute to elucidating the impact that obesity could have on the brain phospho-proteome.