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PXD005780 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRelating human gut metagenome and metaproteome
DescriptionGut microbiome research is rapidly moving towards the functional characterization of the microbiota by means of shotgun meta-omics. Here, we selected a cohort of healthy subjects from an indigenous and monitored Sardinian population to analyze their gut microbiota using both shotgun metagenomics and shotgun metaproteomics. We found a considerable divergence between genetic potential and functional activity of the human healthy gut microbiota, in spite of a quite comparable taxonomic structure revealed by the two approaches. Investigation of inter-individual variability of taxonomic features revealed Bacteroides and Akkermansia as remarkably conserved and variable in abundance within the population, respectively. Firmicutes-driven butyrogenesis (mainly due to Faecalibacterium spp.) was shown to be the functional activity with the higher expression rate and the lower inter-individual variability in the study cohort, highlighting the key importance of the biosynthesis of this microbial by-product for the gut homeostasis. The taxon-specific contribution to functional activities and metabolic tasks was also examined, giving insights into the peculiar role of several gut microbiota members in carbohydrate metabolism (including polysaccharide degradation, glycan transport, glycolysis and short-chain fatty acid production). In conclusion, our results provide useful indications regarding the main functions actively exerted by the gut microbiota members of a healthy human cohort, and support metaproteomics as a valuable approach to investigate the functional role of the gut microbiota in health and disease.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlessandro Tanca
SpeciesList scientific name: human gut metagenome; NCBI TaxID: 408170;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-01-25 02:35:20ID requested
12017-07-19 00:21:23announced
Publication List
Tanca A, Abbondio M, Palomba A, Fraumene C, Manghina V, Cucca F, Fiorillo E, Uzzau S, Potential and active functions in the gut microbiota of a healthy human cohort. Microbiome, 5(1):79(2017) [pubmed]
Keyword List
curator keyword: Metaproteomics, Biological
submitter keyword: gut metagenome, gut metaproteome, metaproteomics
Contact List
Sergio Uzzau
contact affiliationPorto Conte Ricerche, Alghero, Sardinia, Italy
contact emailuzzau@portocontericerche.it
lab head
Alessandro Tanca
contact affiliationProteomics Laboratory, Porto Conte Ricerche, Alghero, Italy
contact emailtanca@portocontericerche.it
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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