PXD005252

PXD005252 is an original dataset announced via ProteomeXchange.

Title	Time-resolved analysis reveals rapid dynamics and broad scope of the CBP/p300 acetylome
Description	The acetyltransferases CBP and p300 are multifunctional transcriptional co-activators; however, their acetylation targets, site-specific acetylation kinetics, and function in proteome regulation are incompletely understood. We combined quantitative proteomics with novel CBP/p300-specific catalytic inhibitors, bromodomain inhibitor, and gene knockout to show that CBP/p300 acetylates thousands of sites, including signature histone sites, as well as a multitude of sites on signaling effectors and enhancer-associated transcriptional regulators. Kinetic analysis identified a subset of CBP/p300-regulated sites with very rapid (<30min) acetylation turnover, revealing a dynamic balance between acetylation and deacetylation. Quantification of acetylation, mRNA, and protein abundance after CBP/p300 inhibition reveals a kinetically competent network of gene expression that strictly depends on CBP/p300-catalyzed rapid acetylation. Collectively, our in-depth acetylome analyses reveal systems attributes of CBP/p300 targets, and the resource dataset provides a framework for investigating CBP/p300 functions, as well as for understanding the impact of small molecule inhibitors targeting its catalytic and bromodomain activities.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:01.622.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brian Weinert
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-02 02:42:28	ID requested
1	2018-06-10 10:55:48	announced
⏵ 2	2024-10-22 04:33:06	announced	2024-10-22: Updated project metadata.

10.1016/j.cell.2018.04.033;

Weinert BT, Narita T, Satpathy S, Srinivasan B, Hansen BK, Sch, ö, lz C, Hamilton WB, Zucconi BE, Wang WW, Liu WR, Brickman JM, Kesicki EA, Lai A, Bromberg KD, Cole PA, Choudhary C, Time-Resolved Analysis Reveals Rapid Dynamics and Broad Scope of the CBP/p300 Acetylome. Cell, 174(1):231-244.e12(2018) [pubmed]

curator keyword: Biological

submitter keyword: CBP, acetyltransferase, acetylation, p300

Chunaram Choudhary
contact affiliation	Proteomics Program Blegdamsvej 3 2200 København N Bygning 6 Building: 06-2-36
contact email	chuna.choudhary@cpr.ku.dk
lab head
Brian Weinert
contact affiliation	Proteomics
contact email	brtw@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD005252

PRIDE project URI

• PRIDE
  1. PXD005252
     1. Label: PRIDE project
     2. Name: Time-resolved analysis reveals rapid dynamics and broad scope of the CBP/p300 acetylome