PXD004894 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry |
Description | Although mutations may represent attractive targets for immunotherapy, direct identification of mutated peptide ligands isolated from human leukocyte antigens (HLA) on the surface of native tumor tissue has so far not been successful. Using advanced mass spectrometry (MS) analysis, we survey the melanoma-associated immunopeptidome to a depth of 95,500 patient-presented peptides. We thereby discover a large spectrum of attractive target antigen candidates including cancer testis antigens and phosphopeptides. Most importantly, we identify peptide ligands presented on native tumor tissue samples harboring somatic mutations. Four of eleven mutated ligands prove to be immunogenic by neoantigen-specific T-cell responses. Moreover, tumor-reactive T cells with specificity for selected neoantigens identified by MS are detected in the patient`s tumor and peripheral blood. We conclude that direct identification of mutated peptide ligands from primary tumor material by MS is possible and yields true neoepitopes with high relevance for immunotherapeutic strategies in cancer. |
HostingRepository | PRIDE |
AnnounceDate | 2017-06-06 |
AnnouncementXML | Submission_2017-06-06_00:30:36.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michal Bassani-Sternberg |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-08-25 00:03:08 | ID requested | |
1 | 2016-11-30 08:33:14 | announced | |
2 | 2017-04-20 06:21:52 | announced | Updated project metadata. |
⏵ 3 | 2017-06-06 00:30:37 | announced | Updated project metadata. |
4 | 2017-10-24 04:03:28 | announced | Updated project metadata. |
5 | 2024-10-22 04:31:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
Bassani-Sternberg M, Br, รค, unlein E, Klar R, Engleitner T, Sinitcyn P, Audehm S, Straub M, Weber J, Slotta-Huspenina J, Specht K, Martignoni ME, Werner A, Hein R, H Busch D, Peschel C, Rad R, Cox J, Mann M, Krackhardt AM, Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry. Nat Commun, 7():13404(2016) [pubmed] |
Keyword List
ProteomeXchange project tag: Human Immuno-Peptidome Project (HUPO-HIPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
curator keyword: Biomedical |
submitter keyword: Immunopeptidomics, LC-MS/MS, cancer antigens, neoantigens |
Contact List
Michal Bassani-Sternberg |
contact affiliation | Head / Immunopeptidomics unit Department of Oncology UNIL/CHUV Ludwig Cancer Research Center Biopole III, Chemin des Boversses 155 Epalinges, 1066 Switzerland |
contact email | michal.bassani@chuv.ch |
lab head | |
Michal Bassani-Sternberg |
contact affiliation | UNIL/CHUV |
contact email | michal.bassani@chuv.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004894
- Label: PRIDE project
- Name: Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry