The amyloid beta (Aβ) peptide represents a 37 to 49 amino acids endoproteolytic fragment of the amyloid precursor protein. The cellular biology that governs the formation and clearance of Aβ has been understood to play a critical role in Alzheimer’s disease (AD). Here we identified by quantitative mass spectrometry proteins in human brain extract that bind to oligomeric Aβ1-42 (oAβ1-42) and/or monomeric Aβ1-42 (mAβ1-42) baits. Whereas several studies are available that interrogated the APP interactome by affinity purification MS, a similar investigation has, to our knowledge, not yet been reported for the Aβ peptide. To address this shortcoming, we have now undertaken an in-depth search for proteins that can bind to Aβ using biotinylated Aβ peptides as baits and human frontal lobe extract as the biological source for the capture of Aβ binding proteins. The study made use of isobaric tagging for the relative quantitation of proteins.