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PXD004367 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrative proteomics and phosphoproteomics profiling and systems biology analysis reveal dynamic signaling networks and mTORC1-dependent mitochondrial function in T cell activation
DescriptionThe underlying mechanisms by which naïve T cells exit from quiescence after antigen stimulation remain elusive. Using multiplex isobaric labeling proteomics technology, we report unbiased, temporal profiling of whole proteome (>8,000 proteins) and phosphoproteome (>13,000 phosphopeptides) during the activation in the wild type and Rptor-/- T cells. TCR stimulation results in dynamic reprogramming of the proteome and phosphoproteome, with predominant upregulation of molecular machineries in protein translation and mitochondrial activation. Loss of mTORC1 disrupts TCR-induced mitochondrial functions including mitoribosome biogenesis, one-carbon metabolism and oxidative phosphorylation.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterxusheng wang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-06-20 02:12:21ID requested
12017-03-15 03:13:05announced
Publication List
Tan H, Yang K, Li Y, Shaw TI, Wang Y, Blanco DB, Wang X, Cho JH, Wang H, Rankin S, Guy C, Peng J, Chi H, Integrative Proteomics and Phosphoproteomics Profiling Reveals Dynamic Signaling Networks and Bioenergetics Pathways Underlying T Cell Activation. Immunity, 46(3):488-503(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: T cell activation, proteomic, phosphoproteomics, mass spectrometry, system biology, mTORC1, mitochondrial function, Rptor
Contact List
Junmin Peng
contact affiliationSt. Jude Children's Research Hospital
contact emailJunmin.Peng@STJUDE.ORG
lab head
xusheng wang
contact affiliationProteomics
contact emailxushengwang78@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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