PXD003841

PXD003841 is an original dataset announced via ProteomeXchange.

Title	Tube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples
Description	Comparison of tube-gel protocol to stacking gel and liquid digestion for label-free extracted ion-current quantification of complex biological samples Dataset with known fold-changes (UPS1 spiked in yeast background at 25-10-5-2.5-1-0.5 fmol)
HostingRepository	PRIDE
AnnounceDate	2016-10-18
AnnouncementXML	Submission_2016-10-18_02:42:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leslie MULLER
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-22 06:23:54	ID requested
⏵ 1	2016-10-18 02:42:43	announced

Muller L, Fornecker L, Van Dorsselaer A, Cianf, é, rani S, Carapito C, Benchmarking sample preparation/digestion protocols reveals tube-gel being a fast and repeatable method for quantitative proteomics. Proteomics, 16(23):2953-2961(2016) [pubmed]

submitter keyword: Label-free quantitative proteomics, sample preparation, tube-gel, stacking gel, liquid digestion

Sarah CIANFERANI
contact affiliation	UMR 7178 - IPHC - CNRS - UdS - Laboratory of BioOrganic Mass Spectrometry - STRASBOURG, FRANCE
contact email	sarah.cianferani@unistra.fr
lab head
Leslie MULLER
contact affiliation	UMR7178 IPHC-DSA-LSMBO
contact email	leslie.muller@etu.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD003841

PRIDE project URI

• PRIDE
  1. PXD003841
     1. Label: PRIDE project
     2. Name: Tube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples