This submission contains the mass spectrometry files for the manuscript by Campbell et al. that describes the characterization of a protein complex containing RNF146 and its respective mutants involved in epithelial polarity. This submission contains 12 DDA files for the following baits: empty 3XFLAG control, GFP-BirA control, RNF146 WT-BirA, and mutants H53A, R163A, and the double mutant H53A_R163A.