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PXD003563 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUltra-deep tyrosine phosphoproteome analysis enabled by an SH2 domain-derived phosphotyrosine superbinder
DescriptionWe present a new strategy for systematic identification of phosphotyrosine (pTyr) by AP-MS using an SH2 domain-derived pTyr-superbinder as the affinity reagent. The superbinder allows for markedly deeper and broader coverage of the Tyr phosphoproteome than conventional anti-phosphotyrosine antibodies when an optimal amount is used. Indeed, we identified approximately 20,000 distinct phosphotyrosyl peptides and more than 10,000 pTyr sites, of which 36% are novel, from 9 cell lines using the superbinder approach. Intriguingly, tyrosine kinases, SH2 domains and phosphotyrosine phosphatases were found preferably phosphorylated, suggesting that the toolkit of kinase signaling is subjected to intensive regulation by phosphorylation. Cell type-specific global kinase activation patterns inferred from label-free quantitation of Tyr phosphorylation guided the design of experiments to inhibit cancer cell proliferation by blocking the highly activated tyrosine kinases. Therefore, the superbinder is a highly efficient and cost-effective alternative to conventional antibodies for systematic and quantitative analysis of the tyrosine phosphoproteome under normal or pathological conditions.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-02-03 02:27:20ID requested
12016-07-11 15:37:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: tyrosine phosphorylation, phosphotyrosine enrichment
Contact List
hanfa Zou; Shawn Li
lab head
contact affiliationresearch officer
contact emailleili.research@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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