PXD002720

PXD002720 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of integrin-associated adhesion complexes isolated from cells treated with DMSO or FAK inhibitor
Description	Human foreskin fibroblast cells were spread on fibronectin (FN) for 1 h and treated with DMSO or FAK inhibitor (3 uM, AZ13256675 (termed AZ675 for short)) for 1 h. Integrin-associated adhesion complexes isolated from these cells were analysed by mass spectrometry to determine changes in adhesion complex composition upon FAK inhibition. As a negative control, complexes were also isolated from cells attached to transferrin (Tf), which allows integrin-independent adhesion via the transferrin receptor.
HostingRepository	PRIDE
AnnounceDate	2016-02-02
AnnouncementXML	Submission_2016-02-15_06:19:32.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002720
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Edward Horton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-13 01:15:46	ID requested
1	2016-02-02 01:14:02	announced
⏵ 2	2016-02-15 06:19:33	announced	Updated publication reference for PubMed record(s): 26833789.

Horton ER, Humphries JD, Stutchbury B, Jacquemet G, Ballestrem C, Barry ST, Humphries MJ, Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition. J Cell Biol, 212(3):349-64(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Focal adhesion kinase (FAK), integrin, adhesion, proteomics, MS

Martin J. Humphries
contact affiliation	Faculty of Life Sciences, Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, United Kingdom
contact email	Martin.Humphries@manchester.ac.uk
lab head
Edward Horton
contact affiliation	University of Manchester
contact email	edward.horton-2@postgrad.manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002720

PRIDE project URI

• PRIDE
  1. PXD002720
     1. Label: PRIDE project
     2. Name: Proteomic analysis of integrin-associated adhesion complexes isolated from cells treated with DMSO or FAK inhibitor