Updated project metadata. The dataset is a concatenation of nanoLC-MS/MS analyses performed on different sample types/sample preparations of human proteins on various instrumental platforms in the laboratory. A franco-swiss collaboration to identify missing human proteins on chromosomes 2 and 14 in the frame of the Human Proteome Project, set up a bioinformatic screen over all datasets accumulated in the laboratory in order to detect proteins classified as “missing” by NextProt.