PXD001903

PXD001903 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of the effect of deletion of PUF3 on yeast via LC-MSMS.
Description	The Puf family of RNA-binding proteins regulate the expression of genes by controlling protein synthesis or stimulating RNA decay. Puf3p is one of six Puf-family members in Saccharomyces cerevisiae. Puf3p has been characterised previously as regulating mRNA decay of nuclear mRNAs that encode for mitochondrial proteins. We undertook a series of genome wide approaches to identify an expanded set of Puf3p target mRNAs and to quantitatively assess the impact of loss of PUF3 on the expression control in actively growing wild type and puf3Δ strains. Here we report our quantifications of relative protein levels. The data suggest that loss of Puf3p only impacts on a small proportion of the proteins encoded by its mRNA targets.
HostingRepository	PRIDE
AnnounceDate	2016-04-13
AnnouncementXML	Submission_2016-04-13_04:23:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001903
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Talavera
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Dionex instrument model; Mascot; Progenesis LC-MS; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-11 06:19:03	ID requested
⏵ 1	2016-04-13 04:23:02	announced

Kershaw CJ, Costello JL, Talavera D, Rowe W, Castelli LM, Sims PF, Grant CM, Ashe MP, Hubbard SJ, Pavitt GD, Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p. Sci Rep, 5():15518(2015) [pubmed]

curator keyword: Biological

submitter keyword: Yeast, Label-free quantification, LC-MSMS

Graham Pavitt
contact affiliation	Faculty of Life Science, University of Manchester, United Kingdom
contact email	graham.pavitt@manchester.ac.uk
lab head
David Talavera
contact affiliation	University of Manchester
contact email	David.Talavera@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD001903

PRIDE project URI

• PRIDE
  1. PXD001903
     1. Label: PRIDE project
     2. Name: Proteomics analysis of the effect of deletion of PUF3 on yeast via LC-MSMS.