PXD001809-1

PXD001809 is an original dataset announced via ProteomeXchange.

Title	Mesenchymal stem cell integrin-associated complexes
Description	The composition and stiffness of the extracellular matrix (ECM) environment that surrounds Multipotent mesenchymal stem cells (MSCs) stem cells dictates transcriptional programming, thereby affecting stem cell lineage decision-making. Cells sense force between themselves and their microenvironment controlling the capability of MSCs to differentiate into adipocytes, osteocytes and chondrocytes. Force sensing is transmitted by integrin receptors and their associated adhesion signalling complexes. To identify regulators of MSC force sensing we sought to catalogue MSC adhesion complex composition. Therefore we isolated integrin-associated adhesion complexes formed in MSCs plated on the ECM ligand fibronectin. We identifed proteins using mass spectrometry that define a MSC specific subset of adhesion complex proteins consisting of key linkages to the actin cytoskeleton together with integrin signalling and force sensing components.
HostingRepository	PRIDE
AnnounceDate	2015-07-20
AnnouncementXML	Submission_2015-07-20_05:31:01.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001809
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jon Humphries
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Velos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-12 08:19:42	ID requested
⏵ 1	2015-07-20 05:31:02	announced

Ajeian JN, Horton ER, Astudillo P, Byron A, Askari JA, Millon-Fr, é, millon A, Knight D, Kimber SJ, Humphries MJ, Humphries JD, Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl, 10(1):51-7(2016) [pubmed]

curator keyword: Biological

submitter keyword: MSC, Integrin, focal adhesions, Extracellular matrix, cell adhesion

Martin J Humphries
contact affiliation	Faculty of Life Sciences, University of Manchester, UK
contact email	martin.humphries@manchester.ac.uk
lab head
Jon Humphries
contact affiliation	The University of Manchester
contact email	j.d.humphries@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD001809

PRIDE project URI

• PRIDE
  1. PXD001809
     1. Label: PRIDE project
     2. Name: Mesenchymal stem cell integrin-associated complexes