Release of neutrophil subsets (hypersegmented, mature and banded) into the bloodstream was evoked by acute inflammation induced by iv. Injection of 2ng/kg lipopolysaccharide in healthy human volunteers pulse (6 hrs) labelled with deuteriated glucose. Three hours after injection, the three neutrophil subsets were sorted from blood. Incorporation of label into DNA was measured and proteome profiles were determined using high-end proteomics techniques (dimethyl labeling, SCX, nano-uplc, Q-Exactive).