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PXD001385 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntensity-based label-free proteomics using a quadrupole orbitrap mass spectrometer
DescriptionWe present a unique data set for benchmarking label free quantitative proteomics using a quadrupole orbitrap mass spectrometer. Soluble Escherichia coli digest was spiked into a HeLa digest in four different concentrations, simulating protein expression differences. The data set, which is available online, provides a unique opportunity to test the proteomic platform (instrumentation and software) in its ability to perform intensity based label free quantification. We show that the presented combination of informatics and instrumentation produces high precision and quantification accuracy.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYishai Levin
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-10-08 03:05:54ID requested
12015-03-25 07:34:03announced
22015-03-27 01:30:53announcedUpdated project metadata.
Publication List
Shalit T, Elinger D, Savidor A, Gabashvili A, Levin Y, MS1-based label-free proteomics using a quadrupole orbitrap mass spectrometer. J Proteome Res, 14(4):1979-86(2015) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Proteomics, Q Exactive, Quadrupole Orbitrap, label-free
Contact List
Yishai Levin
contact affiliationde Botton Institute for Protein Profiling, The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science
contact emailyishai.levin@weizmann.ac.il
lab head
Yishai Levin
contact affiliationWeizmann Institute of Science, Israel
contact emailyishai.levin@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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